Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Chemical composition and biological activities of n-butanol extract of Lepidium sativum L (Brassicaceae) seed

Ouahiba Ait-yahia¹ , Francois Perreau², Samia-Aichouche Bouzroura³, Yamina Benmalek⁴, Tahar Dob⁵, Aicha Belkebir¹

¹Laboratory of Vegetale Physiology, Faculty of Biological Science, Houari Boumediene University of Sciences and Technology, BP 31, El-Alia, Bab-Ezzouar 16111, Algiers, Algeria; ²Institut Jean-Pierre Bourgin, INRA, Agro Paris Tech, CNRS, Universite Paris-Saclay, 78000 Versailles, France; ³Laboratory of Applied Organic Chemistry; ⁴Laboratory of Microbiology, Faculty of Biological Science, Houari Boumediene University of Sciences and Technology, BP 31, El-Alia, Bab-Ezzouar 16111; ⁵Laboratory of Research for Bioactive Products, Ecole Normale SupÃ©rieure (ENS-Kouba), Algiers, Algeria.

For correspondence:- Ouahiba Ait-yahia Email: hiba.ait357@yahoo.com Tel:+213552732833

Accepted: 30 April 2018 Published: 28 May 2018

Citation: Ait-yahia O, Perreau F, Bouzroura S, Benmalek Y, Dob T, Belkebir A. Chemical composition and biological activities of n-butanol extract of Lepidium sativum L (Brassicaceae) seed. Trop J Pharm Res 2018; 17(5):891-896 doi: 10.4314/tjpr.v17i5.20

© 2018 The authors.
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Abstract

Purpose: To investigate the chemical composition, antioxidant properties and antibacterial effects of n-butanol extract of Lepidium sativum (Brassicaceae) seed.

Methods: The antioxidant activity of extracts of the seed obtained by maceration was determined by 1,1-diphenyl-2 picrylhydrazyl (DPPH), reducing power, and β-caroten/linoleic acid assays. The antimicrobial activity of the extract was tested against five strains of pathogenic microorganisms, viz, Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 43300, Enterococcus feacalis ATCC 29212, Escherichia coli ATCC 25992 and Pseudomonas aeruginosa ATCC 27852. Its phytochemical composition was determined by liquid chromatography-mass spectrometry (LC-MS).

Results: A total of 17 compounds were identified by LC-MS, among which four compounds were kaempferol-hexose-rhamnose and three derivatives of kaempferol-rhamnose-hexose (benzoyl hexose) were previously unknown. The extract had better antioxidant than antibacterial properties.

Conclusion: These results indicate that n-butanol extract of Lepidium sativum seeds possesses good antioxidant properties which are most likely due to the presence of quercetin and kaempferol. Thus, these compounds account for the use of Lepidium sativum in traditional medicine.

Keywords: Lepidium sativum, Quercetin, Kaempferol, Antioxidant, Antibacterial